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Claudio Ranieri's AS Roma side suffered a blow to their top-four hopes in Serie A as they went down to a surprise 2-1 defeat at struggling SPAL.
Inter Milan bounced back to beat SPAL 2-0 in Serie A on Sunday and stay on course for a Champions League spot next season.
Sampdoria's in-form veteran forward Fabio Quagliarella scored two early goals to lead his side to a 2-1 win at SPAL.
A controversial decision from a Video Assistant Referee (VAR) review proved crucial as Fiorentina overcame SPAL 4-1 in Serie A on Sunday at the Stad
Torino coach Walter Mazzarri was left fuming with the refereeing after Nicolas N'Koulou was sent off during his side's 0-0 draw at SPAL.
AC Milan ended a four-match goal drought and striker Gonzalo Higuain was on target for the first time in two months as they beat SPAL on Saturday.
Goals either side of the break from the Portugal international and Mario Mandzukic earned Juve their 12th win of an unbeaten start in the league.