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A late Lorenzo Insigne goal rescued a 1-1 draw for second-placed Napoli at Sassuolo but it was not enough to stop them losing ground on Juventus.
AC Milan leapfrogged rivals Inter Milan into third place in Serie A on Saturday with a 1-0 win over 10-man Sassuolo at San Siro.
Cristiano Ronaldo scored his 18th Serie A goal of the season as Juventus claimed a 3-0 win over Sassuolo to open up an 11-point lead over Napoli.
Barcelona have signed Sassuolo's Kevin-Prince Boateng on loan until the end of the season, the club announced on Monday.
Inter Milan's poor run against Sassuolo continued on Saturday as they were held to a 0-0 home draw in Serie A in front of a crowd of school kids.
Inter Milan are due to serve the first of their two-match stadium closure issued following fans' racial abuse of Kalidou Koulibaly against Sassuolo.
Napoli striker Arkadiusz Milik scored one goal and provided an assist for another as his side beat Sassuolo 2-0 in a Coppa Italia round of 16 match.
Atalanta forward Josip Ilicic came off the bench to score a hat trick in the last 20 minutes in a 6-2 victory over Sassuolo.
Nicolo Zaniolo scored his first-ever Serie A goal in style on Wednesday with a stunning solo effort to cap AS Roma's win over Sassuolo.