Sporting Kansas City overturned a first-leg deficit to beat Independiente de la Chorrera and advance to the CONCACAF Champions League semis.
Frank de Boer's side won on the night but went out of its quarterfinal with Monterrey on aggregate, another blemish for the defending MLS champions.
Liga MX got the better of MLS as Santos Laguna quashed New York's brief moment of hope on Tuesday night and Tigres eased past Houston.
The reigning MLS champions fell to Monterrey 3-0 in their quarterfinal, and Sporting Kansas City lost 2-1 to upstart Independiente of Panama.
Mexico's Santos Laguna claimed a 2-0 win over the New York Red Bulls, and UANL won by the same score over Houston, in the CONCACAF Champions League.
Atlanta United overturned a two-goal deficit, to advance to the quarterfinals of the CONCACAF Champions League, while Sporting KC saw off Toluca.
New York Red Bulls set up an MLS-Liga MX clash with Santos Laguna as both sides advanced in the CONCACAF Champions League match.
Toronto FC made a quick exit from the CCL on Tuesday with a 1-1 home draw against Independiente that sealed the Panamanian team's 5-1 aggregate win.
Sporting KC won 3-0 over Liga MX side Toluca in the CONCACAF Champions League on Tuesday, while Atlanta United fell 3-1 to Costa Rica's Herediano.
An own goal and a Daniel Royer tally gave NYRB a 2-0 win over Atletico Pantoja in the first leg of their CCL opening-round series on Wednesday.
The Dominican powerhouse has barely been in existence for 20 years but has a chance to imprint its next footprint in the CONCACAF Champions League.
Panamanian side Club Atletico Independiente shocked Toronto FC in the first leg of the CONCACAF Champions League round of 16.
Tigres, Monterrey and Santos Laguna face managable Round of 16 opponents in the tournament, but Toluca have a stern MLS test against Sporting KC.
Hirving Lozano's goal wasn't enough for PSV Eindhoven to advance, but Hector Herrera helped Porto reach the next stage of Champions League.
MLS Cup host Atlanta has been drawn with Costa Rican opposition, while Portland will meet opponents from Dominican Republic if they win on Saturday.
Jozy Altidore scored a hat trick to help Toronto FC win the Canadian Championship for a third consecutive year with a 7-4 aggregate triumph.
Mexico have won CONCACAF's regional club tournament 13 straight times and a record 33 times overall. How does that dominance compare globally?
Chivas boss Matias Almeyda has repeatedly proven his managerial credentials in Mexico. After winning the CCL will Mexico come calling?
Chivas coach Matias Almeyda won the competition after a trying season in which players have not been paid bonuses and transfers were few.
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