Bibiana Steinhaus became the first female referee in the Bundesliga when she oversaw the 1-1 draw between Werder Bremen and Hertha Berlin on Sunday.
The 38-year-old, who is also a police officer, joined Germany's leading division in May following six years in the second tier.
She officiated Bayern Munich's first-round DFB Pokal defeat of Chemnitzer in August, the Women's Champions League final earlier this year, and also at Euro 2017.
The development follows Lorraine Watson becoming the first female referee in Scottish men's football on Saturday, when League Two Edinburgh City defeated Berwick 1-0.
Matthew Leckie's 39th-minute goal had given Hertha a half-time lead, but Thomas Delaney equalised for Werder 11 minutes into the second half.