Speaking in an interview on Saturday, De Ligt said: "I have not taken a vaccination. It is not mandatory. I think you should be in charge of your own body.
"The risk of infection is always there. I try to come into contact with as few people as possible outside the Dutch national team."
"In my recent interview, I was not clear with my response," he wrote on Twitter. "In order to clear any doubts: I am absolutely in favour of the COVID-19 vaccination and will take it as soon as possible."
In my recent interview, I was not clear with my response. In order to clear any doubts: I am absolutely in favour of the COVID-19 vaccination and will take it as soon as possible.— Matthijs de Ligt (@mdeligt_04) May 29, 2021
De Ligt, 21, tested positive for COVID-19 in January and missed two Serie A matches as a result.
The defender has endured a difficult time at Juve this season as the team failed to claim a 10th successive league title, with Inter Milan crowned champions.
"It was a tough season physically and mentally," he added. "A season with ups and downs. I was out for three months, and then come back and you get corona.
"If you had said three weeks ago: 'We will win the cup and we will make Champions League football,' that would have been good."
De Ligt has also been linked with a move away from Turin this summer, but when asked if he could see himself at Barcelona in the future, he replied: "I am very happy at Juve.
"I feel like a fish in water. Despite the team performances being less, I feel good on the pitch and appreciated. Would they let me go? There are plenty of clubs that have money problems, but you should ask the club."