Sir Bradley Wiggins and Great Britain had to settle for silver in the men's team pursuit on day two of the Track Cycling World Championships on Thursday night as familiar foes Australia claimed gold.
Wiggins, Andy Tennant, Owain Doull and Ed Clancy finished in three minutes 53.856 seconds as Australia's Sam Welsford, Michael Hepburn, Callum Scotson and Miles Scotson won in 3mins 52.727secs. Denmark took bronze.
Britain had trailed for much of the four kilometres, but with two laps of the 16 remaining took the lead, only to unravel in the closing stages.
Clancy had back surgery last year and is still recovering, meaning Britain have more to give as they look for a third straight Olympic title in Rio this summer.
Earlier, Laura Trott won Britain's first gold of the competition in the women's scratch race and Becky James claimed bronze in the women's Keirin.
Two-time Olympic champion Trott put the disappointment of fifth-place in team pursuit qualifying earlier on Thursday behind her to win the non-Olympic discipline.
Trott finished ahead of Kirsten Wild of Holland and Canada's Stephanie Roorda.
It was Trott's sixth world title and second individual honour. Her only prior individual win was in the omnium at the 2012 World Championships in Melbourne.
James was third in the Keirin, which begins behind a motorised Derny bike, behind Germany's Kristina Vogel and Anna Meares of Australia.
James has had two years disrupted by a knee injury and illness since winning the Keirin and individual sprint at the 2013 World Championships in Minsk.
The result represented a major boost for her Rio Olympic hopes as James is not yet at the level she was in Belarus three years ago.